protein level expression  (Human Protein Atlas)

Journal: Translational Oncology

Article Title: Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine

doi: 10.1016/j.tranon.2025.102617

Figure Lengend Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay. Scale bar, 200 μm (J-K) Migration ability of TPC-1 cells after APOC1 knockdown treatment for 24 h as detected by a wound healing assay. (L) Colony formation assay performed on TPC-1 and B-CPAP cells after APOC1 siRNA treatment. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control.

Article Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay.

Techniques: Expressing, Knockdown, CCK-8 Assay, Western Blot, EdU Assay, Migration, Wound Healing Assay, Colony Assay, Control

Journal: Translational Oncology

Article Title: Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine

doi: 10.1016/j.tranon.2025.102617

Figure Lengend Snippet: Expression of APOC1 in PTC and its clinical prevalence. (A-C) Stage-plot analysis of the relationship between APOC1 expression and PTC tumor stages. (D) Age. (E) APOC1 expression in different PTC histological subtypes. (F) Neoplasm locations. (G) Primary neoplasm focus types. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control.

Article Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay.

Techniques: Expressing, Control

Journal: Translational Oncology

Article Title: Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine

doi: 10.1016/j.tranon.2025.102617

Figure Lengend Snippet: Correlation analysis of APOC1 expressions with immune cell infiltration levels and immune checkpoints in PTC. ( A–M ) Correlation between APOC1 expression and infiltration levels of immune cells. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control.

Article Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay.

Techniques: Expressing, Control

Journal: Translational Oncology

Article Title: Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine

doi: 10.1016/j.tranon.2025.102617

Figure Lengend Snippet: Molecular docking visualization of APOC1 and drug candidates. ( A ) Farnesylthioacetic-acid; ( B ) Papaverine; ( C ) Hycanthone; ( D ) Indolophenanthridine; ( E ) Nicaraven; ( F ) Methylnorlichexanthone; ( G ) Fananserin, and ( H ) Cyclopamine.

Article Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay.

Techniques:

Journal: Translational Oncology

Article Title: Apolipoprotein C1 functions as a target of thyroid carcinoma and synergistic effects with promising candidate-cyclopamine

doi: 10.1016/j.tranon.2025.102617

Figure Lengend Snippet: APOC1 deficiency enhances effects of Cyclopamine, inhibits proliferation, and promotes cell death in PTC. ( A ) Cell death after APOC1 knockdown or treatment with 10 μM Cyclopamine for 24 h as analyzed by flow cytometry. ( B ) Colony-forming potential of tumor cells in B-CPAP and TPC-1 cells with APOC1 knockdown or treatment with 10 μM Cyclopamine for 14 days. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control.

Article Snippet: APOC1 deficiency inhibits aggressive PTC progression. (A-C) Analyses of APOC1 in PTC tissues (T) and adjacent normal tissues (N). (D) APOC1 protein expression levels in normal and tumor thyroid gland tissues obtained from the Human Protein Atlas database. (E) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by qPCR. (F) CCK8 assay of indicated knockdown APOC1 treatments in TPC-1 and B-CPAP cells. (G) Knockdown efficiencies of APOC1 siRNA into TPC-1 and B-CPAP cells as evaluated by Western blot. (H-I) TPC-1 cell proliferation after APOC1 knockdown treatments for 24 h as detected by the EdU assay.

Techniques: Knockdown, Flow Cytometry, Control